| 2024-05-12 14:30 | J33 | FC Twente |  | - |  | FC Volendam | | FF |
| 2024-05-11 16:30 | J22 | FC Twente | | - |  | Telstar | | FF |
| 2024-04-24 18:45 | J31 | FC Twente | | 3-1 |  | Almere City FC | Mathias Kjolo 10', Sem Steijn 52', Daan Rots 84'; Kornelius Hansen 73' | |
| 2024-04-06 21:00 | J29 | FC Twente | | 2-0 |  | Fortuna Sittard | Ricky van Wolfswinkel 86'; Sadik Fofana 52' (p.p.) | |
| 2024-03-31 14:30 | J27 | FC Twente | | 1-0 |  | Heracles Almelo | Myron Boadu 54' | |
| 2024-03-30 14:00 | J19 | FC Twente | | 1-0 |  | ADO Den Haag | Marit Auée 13' | |
| 2024-03-10 16:45 | J17 | FC Twente | | 3-1 |  | PSV | Ella Peddemors 28', Elena Dhont 81' 87'; Joëlle Smits 62' | |
| 2024-03-09 18:45 | J25 | FC Twente | | 2-1 |  | Sparta Rotterdam | Daan Rots 21', Myron Boadu 77'; Pelle Clement 87' | |
| 2024-03-03 12:15 | J16 | FC Twente | | 0-3 |  | Ajax | Romée Leuchter 25', Nadine Noordam 38', Jonna van de Velde 72' | |
| 2024-02-25 14:30 | J23 | FC Twente | | 3-0 |  | Go Ahead Eagles | Sem Steijn 12' 36', Joshua Brenet 60' | |
| 2024-02-18 12:15 | J22 | FC Twente | | 0-1 |  | FC Utrecht | Jens Toornstra 29' | |
| 2024-02-04 12:15 | J14 | FC Twente | | 5-2 |  | Excelsior | Taylor Ziemer 5', Wieke Kaptein 15', Danique Ginkel 25', Kim Everaerts 33', Eva Elberink 59'; Sabrine Ellouzi 55', Lynn Groenewegen 90' | |
| 2024-02-03 16:30 | J20 | FC Twente | | 3-0 |  | RKC Waalwijk | Ricky van Wolfswinkel 21', Myron Boadu 79', Younes Taha El Idrissi 81' | |
| 2024-01-13 18:45 | J17 | FC Twente | | 2-1 |  | AZ Alkmaar | Manfred Ugalde 42' 70'; Jens Odgaard 4' | |
| 2023-12-17 16:45 | J1 | FC Twente | | 3-2 |  | Heerenveen | Leonie Vliek 12', Taylor Ziemer 33' 53'; Janneke Ennema 23', Lyanne Iedema 34' | |
| 2023-12-13 18:45 | J5 | FC Twente | | 2-1 |  | Zwolle | Liz Rijsbergen 59' 83'; Jasmijn Dijsselhof 19' | |
| 2023-12-10 16:45 | J11 | FC Twente | | 2-1 | | AZ Alkmaar | Taylor Ziemer 11', Marit Auée 24'; Desiree van Lunteren 88' | |
| 2023-12-08 20:00 | J15 | FC Twente | | 4-2 | | Excelsior | Manfred Ugalde 45', van Wolfswinkel 59' (p.) 80', Younes Taha El Idrissi 85'; Troy Parrott 9', Sven Nieuwpoort 13' | |
| 2023-11-25 18:45 | J13 | FC Twente | | 0-3 | | PSV | Robin Propper 49' (p.p.); Joey Veerman 45', Johan Bakayoko 82' | |
| 2023-11-24 19:30 | J10 | FC Twente | | 4-0 | | FC Utrecht | Ella Peddemors 27', Liz Rijsbergen 72', Taylor Ziemer 81'; Ilse van der Zanden 75' (p.p.) | |
| 2023-11-11 20:00 | J12 | FC Twente | | 3-3 |  | NEC | Sem Steijn 43' 76', Manfred Ugalde 87'; Mathias Ross 47', Bart van Rooij 56', Youri Baas 65' | |
| 2023-11-05 12:15 | J7 | FC Twente | | 3-1 | | Fortuna Sittard | Marisa Olislagers 22', Taylor Ziemer 40', Leonie Vliek 61'; Tessa Wullaert 62' | |
| 2023-10-29 12:15 | J10 | FC Twente | | 2-1 |  | Feyenoord | Manfred Ugalde 10', van Wolfswinkel 78'; Lutsharel Geertruida 86' | |
| 2023-10-18 19:00 | 2E | FC Twente | | 1-2 |  | BK Hacken | Leonie Vliek 33'; Anna Anvegård 61', Felicia Schröder 65' | |
| 2023-10-01 12:15 | J3 | FC Twente | | 3-1 | | Feyenoord | Taylor Ziemer 4', Liz Rijsbergen 11' 50'; Noele van der Sluijs 43' | |
| 2023-09-30 21:00 | J7 | FC Twente | | 1-0 | | Heerenveen | Michel Vlap 65' | |
| 2023-09-27 20:00 | J3 | FC Twente | | 1-0 |  | Vitesse | Sem Steijn 39' | |
| 2023-09-17 14:30 | J5 | FC Twente | | 3-1 | | Ajax | Daan Rots 7', Sem Steijn 11', Naci Unuvar 79'; Brian Brobbey 35' | |
| 2023-08-31 19:00 | POff | FC Twente | | 0-1 |  | Fenerbahçe | Edin Dzeko 72' (p.) | |
| 2023-08-20 14:30 | J2 | FC Twente | | 3-1 | | Zwolle | Michal Sadílek 18', Sem Steijn 76'; Ferdy Druijf 33' (p.), Jasper Schendelaar 90' (p.p.) | |
| 2023-08-10 20:00 | 3E | FC Twente | | 2-0 |  | Riga FC | Alfons Sampsted 11', Daan Rots 67' | |
| 2023-07-27 20:00 | 2E | FC Twente | | 1-0 |  | Hammarby | Sem Steijn 53' | |
